To examine the functional effect of NKG2D, there are two types of human large intestinal microvascular endothelial cells are used that are given below:
- Human intestinal microvascular endothelial cells
- Human large intestinal microvascular endothelial cells
Among these, the human large intestine microvascular endothelial cells are the primary cells that were originated from the tissues of the normal large intestine. These cells are negative for HBV, HIV-1, mycoplasma, and HCV. According to the information provided by the manufacturer, these cells were passaged and grown in CSC complete medium.
The human large intestinal microvascular endothelial cells were used between a passage of 7 and 9 and stimulated at the confluence in 24 well plates. The HLIMEC shows the high endogenous as compared to the HIMEC.
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